Protein synthesis in rabbit reticulocytes. Characteristics of mRNA (AUG codon)-dependent binding of Met-tRNAfMet to 40 S and 80 S ribosomes.
نویسندگان
چکیده
منابع مشابه
synthesis of sulfides from alcohols and thiols in solvent-freeconditions and deoxygenation of sulfoxides
کاتالیست یک سنتز جدید برای تیواترها توصیف شده است. واکنش الکل ها با آریل، هتروآریل و آلکیل تیو ل ها درحضور 1،3،5- تری آزو- 2،4،6- تری فسفرین-2،2،4،4،6،6 هگزاکلراید ((tapc به عنوان یک کاتالیست موُثر، بازده های خوب تا عالی از تیواترها را حاصل می کند. علاوه براین، واکنش تحت شرایط بدون فلز و بدون حلال پیش می رود، بنابراین یک مکمل جالب برای روش های شناخته شده سنتز تیواترها ارائه می دهد. یک مکانیسم ا...
15 صفحه اولProtein synthesis in rabbit reticulocytes: Characteristics of a ribosomal factor that reverses inhibition of protein synthesis
A ribosomal salt (0.5 M KCI) wash factor (RF) that reverses inhibition of protein synthesis in heme-deficient reticulocyte lysates has been resolved from the bulk of MettRNAfmet-binding factor (EIF-1), Co-EIF-I, and EIF-2 (ternary complex dissociation factor, TDF). The purified RF restores protein synthesis activity of heme-deficient lysates to the level observed in the presence of hemin. No di...
متن کاملProtein synthesis in rabbit reticulocytes. Preparation of homogeneous Met-tRNAf deacylase and studies of its role in protein synthesis.
Met-tRNAf deacylase from reticulocyte ribosomes has been purified to homogeneity. Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the homogeneous preparation gives a single protein band corresponding to a molecular weight of approximately 67,000. Purified Met-tRNAf deacylase degrades free Met-tRNAf and also Met-tRNAf bound to 40 S ribosomes in the presence of AUG codon but does ...
متن کاملPreparation and characterization of 80 S ribosomes of ascites tumor cells active in polypeptide synthesis.
The preparation of tumor ribosomes that are essentially free of polyribosomes and active in polypeptide synthesis is described. Most active preparations were obtained by incubating ribosomal suspensions 20 min under conditions of protein synthesis. After this time, all polysomes had been converted to 80 S particles which could be stimulated to polypeptide synthesis by polyuridylic acid or liver...
متن کاملBinding of Met-tRNAf to native 40 S ribosomal subunits in Ehrlich ascites tumor cells.
Two forms of native 40 S ribosomal subunits, distinguishable by their buoyant densities, are recovered from Ehrlich ascites cells. In this communication, we describe experiments designed to test whether Met-tRNAf is associated with either form. Our results indicate that (a) in the cell, Met-tRNAf is bound to the native 40 S subunit, and in particular, to the subunit of density 1.40 g x cm-3; (b...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1976
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(20)81872-9